Figure 1.

EPO does not increase myelin gene expression in wild-type CG4 cells. CG4 cells were plated in poly-l-ornithine–coated 24 well plates in growth medium (GM). After 2 d, cells were switched to differentiation medium (DM) and cultured in the absence or in the presence of EPO 80 ng/mL. At d 5 of differentiation MOG (A) and MBP (B) gene expression were analyzed by quantitative PCR (qPCR). Results are expressed as arbitrary units versus undifferentiated (UND) cells and are the mean ± standard deviation (SD) of six samples from two independent experiments analyzed in duplicate. ***P < 0.001 versus UND cells by two-tailed unpaired Student t test.