The chordoma cells were cultured in different commercial available tissue culture media and collagen substrate, and their proliferate activity was evaluated by MTT and numerication assay. A, MTT assay indicated that the CH 8 cells proliferated less actively in RPMI medium and more actively in DMEM and IMDM media. B, MTT assay indicated that the GB 60 cells proliferated less actively in RPMI medium and more actively in DMEM and IMDM media. C, MTT assay indicated that the U-CH1 cells proliferated less actively in RPMI medium and more actively in DMEM medium. D, Cell numeration assay indicated that all 3 chordoma cell lines universally grew better in collagen substrate than collagen free plate.