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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: J Phys Chem B. 2013 Jul 18;117(30):8987–8993. doi: 10.1021/jp4052598

Figure 4.

Figure 4

Coomassie blue stained tris-glycine SDS-PAGE illustrating efficient, site-specific incorporation of 2 and 3 with high fidelity into sfGFP. The protein constructs were expressed from pBad-sfGFP (wt-sfGFP, lane 2); pBad-sfGFP-75TAG and pDulepN3/pN3CH2Phe (lanes 3 – 5) in the presence (lanes 3 and 4) or absence (lane 5) of 2 or 3, respectively; or pBad-sfGFP-134TAG and pDule-pN3/pN3CH2Phe (lanes 6 – 8) in the presence (lanes 6 and 7) or absence (lane 8) of 2 or 3, respectively.