Table 1.
Overview of the reactions performed showing yields of products formed and substrate recovery rates under different reaction conditions.
| Reagent | pH | Solvent/reagent added | Hydrolysis product | Hydrolysis yield (%) | Substrate recovery rate (%) |
|---|---|---|---|---|---|
| Pancreatin | 4.0 | toluene | isosteviol | 93.9 | 0 |
| Pancreatin | 4.0 | ethanol | isosteviol | 12.4 | 76.0 |
| Pancreatin | 7.0 | toluene | steviol | 20.2 | 51.5 |
| Pancreatin | 7.0 | ethanol | 0 | 0 | 97.6 |
| Pancreatic lipase | 4.0 | toluene | 0 | 0 | 98.4 |
| Pancreatic lipase | 4.0 | ethanol | steviol | 5.2 | 82.2 |
| Pancreatic lipase | 7.0 | toluene | 0 | 0 | 97.3 |
| Pancreatic lipase | 7.0 | ethanol | isosteviol | 4.2 | 80.4 |
| Fungal lipase | 4.0 | toluene | 0 | 0 | 96.7 |
| Fungal lipase | 4.0 | ethanol | steviol | 10.0 | 81.2 |
| Fungal lipase | 7.0 | toluene | 0 | 0 | 97.1 |
| Fungal lipase | 7.0 | ethanol | 0 | 0 | 97.0 |
| A. niger | 7.0 | none | steviol | 20.8 | 38.1 |
| R. arrhizus | 7.0 | none | 0 | 0 | 75.9 |
| R. stolonifer | 7.0 | none | 0 | 0 | 63.2 |
| S. cerevisiae | 7.0 | glucose | 0 | 0 | 26.2 |
| S. cerevisiae | 7.0 | none | 0 | 0 | 0 |