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. 2009 Jun 1;40(2):248–253. doi: 10.1590/S1517-83822009000200008

Table 3.

Year average rainfall (mm3) and relative humidity (RH %) in the turkey producer region: detection of viral RNA (TAstV-2 and TCoV) from CS and faeces searched by simplex and multiplex RT-PCR assays.

Simplex RT-PCRa Multipex RT-PCRb
TAstV-2 TCoV TAstV-2 TCoV
Summerc Winter Summer Winter Summer Winter Summer Winter χ2 p-values OR
CS Positive 200d 250 10 190 300 50 100 100 0.87654 0.67
% 40 50 2 38 60 10 20 20
CS Negative 25e 25 100 200 100 50 80 220 0.67851
% 5 5 20 40 20 10 16 44
Faeces Positive 300 200 150 250 380 20 100 50 0.89982 3.98
% 60 40 30 50 76 4 20 10
Faeces Negative 0 0 20 80 50 50 300 50 0.56743
% _ _ 4 16 10 10 60 10
a-

simplex RT-PCR performed as described in material and methods section;

b-

Multiplex RT-PCR performed as described in material and methods section;

c-

Year average rainfall and humidity year 2006 250mm3 and 78% (summer); 0mm3 and 40% (winter);

d-

Positive results related to total samples analyzed described in material and methods section;

e-

Negative results related to total samples analyzed described in material and methods section.