Table 3.
Year average rainfall (mm3) and relative humidity (RH %) in the turkey producer region: detection of viral RNA (TAstV-2 and TCoV) from CS and faeces searched by simplex and multiplex RT-PCR assays.
| Simplex RT-PCRa | Multipex RT-PCRb | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| TAstV-2 | TCoV | TAstV-2 | TCoV | |||||||
| Summerc | Winter | Summer | Winter | Summer | Winter | Summer | Winter | χ2 p-values | OR | |
| CS Positive | 200d | 250 | 10 | 190 | 300 | 50 | 100 | 100 | 0.87654 | 0.67 |
| % | 40 | 50 | 2 | 38 | 60 | 10 | 20 | 20 | ||
| CS Negative | 25e | 25 | 100 | 200 | 100 | 50 | 80 | 220 | 0.67851 | |
| % | 5 | 5 | 20 | 40 | 20 | 10 | 16 | 44 | ||
| Faeces Positive | 300 | 200 | 150 | 250 | 380 | 20 | 100 | 50 | 0.89982 | 3.98 |
| % | 60 | 40 | 30 | 50 | 76 | 4 | 20 | 10 | ||
| Faeces Negative | 0 | 0 | 20 | 80 | 50 | 50 | 300 | 50 | 0.56743 | |
| % | _ | _ | 4 | 16 | 10 | 10 | 60 | 10 | ||
simplex RT-PCR performed as described in material and methods section;
Multiplex RT-PCR performed as described in material and methods section;
Year average rainfall and humidity year 2006 250mm3 and 78% (summer); 0mm3 and 40% (winter);
Positive results related to total samples analyzed described in material and methods section;
Negative results related to total samples analyzed described in material and methods section.