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Detection level of the PCR assay (Direct and Enriched) as compared to the conventional culture, using serial dilutions of Shigella flexneri spiked to lettuce samples.
| Inoculum Shigella (CFU/sample) | Multiplex PCR positive results | Culture positive results | |
|---|---|---|---|
| Enriched-PCR samples | Direct-PCR samples | ||
| 107 | 3/3 | 0/3 | 10/10 |
| 106 | 3/3 | 0/3 | 5/10 |
| 105 | 10/10 | 0/3 | 1/10 |
| 104 | 10/10 | 0/3 | 0/3 |
| 103 | 0/10 | 0/3 | 0/3 |
| 102 | 0/3 | 0/3 | 0/3 |
| 10 | 0/3 | 0/3 | 0/3 |
| Noninoculated | 0/10 | 0/3 | 0/10 |
