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. 2011 Jun 1;42(2):778–785. doi: 10.1590/S1517-838220110002000046

Table 2.

Modifications in the reagent concentrations and volumes of the PCR reactions (ftsZ I-VIII) used to detect Wolbachia in Anastrepha with the ftsZ primer.

PCR reaction Reagents Concentration Volume (µl)
ftsZ I DNA 50 ng/μl 3.00*
ftsZ II MgCl2 50 mM 0.50*
DNA 50 ng/μl 3.00
ftsZ III Primer F 8 μM* 0.50
Primer R 8 μM* 0.50
DNA 50 ng/μl 3.00*
ftsZ IV MgCl2 50 mM 0.50*
Primer F 8 μM* 0.50
Primer R 8 μM* 0.50
DNA 50 ng/μl 3.00
ftsZ V DNTP 10 mM 0.50*
MgCl2 50 mM 0.50*
DNA 50 ng/μl 3.00*
ftsZ VI DNTP 10 mM 0.50*
MgCl2 50 mM 0.50*
Primer F 8 μM* 0.50
Primer R 8 μM* 0.50
DNA 50 ng/μl 3.00*
ftsZ VII Tag DNA polymerase 1 unit 0.25*
DNA 50 ng/μl 3.00
ftaZ VIII Fresh DNA* 50 ng/μl 5.00
*

Alteration in PCR conditions (reagent concentration and/or volume).