Table 2.
PCR reaction | Reagents | Concentration | Volume (µl) |
---|---|---|---|
ftsZ I | DNA | 50 ng/μl | 3.00* |
ftsZ II | MgCl2 | 50 mM | 0.50* |
DNA | 50 ng/μl | 3.00 | |
ftsZ III | Primer F | 8 μM* | 0.50 |
Primer R | 8 μM* | 0.50 | |
DNA | 50 ng/μl | 3.00* | |
ftsZ IV | MgCl2 | 50 mM | 0.50* |
Primer F | 8 μM* | 0.50 | |
Primer R | 8 μM* | 0.50 | |
DNA | 50 ng/μl | 3.00 | |
ftsZ V | DNTP | 10 mM | 0.50* |
MgCl2 | 50 mM | 0.50* | |
DNA | 50 ng/μl | 3.00* | |
ftsZ VI | DNTP | 10 mM | 0.50* |
MgCl2 | 50 mM | 0.50* | |
Primer F | 8 μM* | 0.50 | |
Primer R | 8 μM* | 0.50 | |
DNA | 50 ng/μl | 3.00* | |
ftsZ VII | Tag DNA polymerase | 1 unit | 0.25* |
DNA | 50 ng/μl | 3.00 | |
ftaZ VIII | Fresh DNA* | 50 ng/μl | 5.00 |
Alteration in PCR conditions (reagent concentration and/or volume).