Table 2.
Level combinations of the two independent variables (temperature and SF concentration) used in the 22 central composite design, and results of the selected responses (clavulanic acid and biomass concentrations).
| Independent variables | * Responses | |||
|---|---|---|---|---|
| Run | Temperature (°C) | SF (g/L) | Clavulanic acid (mg/L) | Biomass (g/L) |
| 14 (C) | 30 | 30 | 567 | 3.4 |
| 7 | 26 | 30 | 501 | 2.4 |
| 12 (C) | 30 | 30 | 566 | 3.3 |
| 10 (C) | 30 | 30 | 567 | 3.1 |
| 5 | 30 | 10 | 221 | 1.8 |
| 6 | 30 | 50 | 582 | 3.9 |
| 2 | 32 | 20 | 377 | 2.0 |
| 13 (C) | 30 | 30 | 567 | 3.5 |
| 11 (C) | 30 | 30 | 568 | 3.4 |
| 4 | 32 | 40 | 629 | 3.3 |
| 9 (C) | 30 | 30 | 565 | 3.1 |
| 8 | 34 | 30 | 595 | 2.9 |
| 3 | 28 | 40 | 562 | 3.0 |
| 1 | 28 | 20 | 306 | 1.3 |
*
All values were statistically significant at 95% confidence level.