Figure 3.

Angiogenic factor-induced phosphorylation and activation of Tpl2. VEGF or CXCL1 induced the phosphorylation of Tpl2 in a time- and dose-dependent manner. (A) Cells were exposed to VEGF or (B) CXCL1 and harvested at the indicated time. Levels of Tpl2 or GAPDH proteins were determined by Western blot analysis at the indicated concentration. (C) VEGF or CXCL1 induced Tpl2 kinase activity in a time-course response. (D) VEGF or CXCL1 induced Tpl2 kinase activity in a dose-dependent manner. Cells were disrupted and kinase activity of Tpl2 was determined as described in the Materials and Methods section. All data are presented as means ± SEM of five independent experiments. *P < .05 compared to controls.