Figure 4.

Human RMS tumors show alterations in p53 tumor suppressor pathway. Tissue lysates of four RMS tumors and their matched normal samples were examined for protein levels of p53 (DO-1; Santa Cruz Biotechnology) and its downstream transcriptional targets p21 (F-5; Santa Cruz Biotechnology) and Bax (B-9; Santa Cruz Biotechnology). β-Actin (AC-15; Sigma) was used as a loading control. Of the four paired samples, p53 is overexpressed in all tumor tissues when compared to corresponding normal tissue (T, tumor tissues; N, normal muscle), although an up-regulation of both p21 and Bax is seen in only one of the four tumors.