Figure 4.

mTOR inhibition induces dormancy and drug resistance in PCa cells. (A) PC3 or (B) C42B cells were treated with 10 nM rapamycin for 0, 24, 48, and 96 hours. The dormant population was examined by flow cytometry using Ki67 antibody. (C) PC3 cells or (E) C42B cells were treated with the indicated anticancer drugs for 2, 3 and 4 days in the presence and absence of rapamycin. Cell death was measured by trypan blue assay. (D) PC3 cells or (F) C42B cells were treated with the indicated anticancer drugs for 72 hours in the presence and absence of rapamycin. Cell death was measured by flow cytometry with Annexin V staining. All results represent average mean ± SD values from triplicate assays, and the experiments were performed twice.