Figure 6.

TBK1 is important for PCa stem-like cells and drug resistance. PC3 cells were infected with lentivirus containing scrambled or TBK1 shRNA plasmid, selected with puromycin, and then cultured for 3 days in the presence and absence of rapamycin. The CD133⁺/CD44⁺ (A and B) and CD133^-/CD44^- (C and D) cells were analyzed by flow cytometry. (E) PC3 cells containing TBK1 or scramble shRNA were treated for 72 hours with taxotere in the presence and absence of rapamycin. Cell death was measured by the trypan blue assay. All results represent mean ± SD values from triplicate assays, and the experiments were performed twice.