Figure 3.

Downregulation of Human SENP1 and ANP32D Orthologs in Drosophila Enhances Survival under Hypoxic Condition

da-Gal4 driver was used for ubiquitously knocking down the candidate gene by crossing with respective UAS-RNAi lines. Respective eclosion rates were observed at 21% and 5% O₂ (Table S2).

(A) CG32110-RNAi when crossed with da-Gal4 significantly increases the eclosion rate at 5% O₂. The results were consistent in three RNAi lines targeting the same human SENP1 ortholog gene CG32110 (^∗p < 0.005, unpaired t test).

(B) The differences were also significant for eclosion rates (%) of the F1 progeny for the two Mapmodulin-RNAi fly lines (^∗p < 0.005, unpaired t test).

Each bar represents mean ± SEM of eclosion rate. The w1118 and da-Gal4 stocks were tested and used as background controls. The genotypes of each RNAi line are provided in Table S4.