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(A and B) Subcellular fractionation of control and Sirt5 KD HEK 293T cells (A) and WT and Sirt5 KO mouse livers
(B). Fractionation controls were SDHA and SOD2 (mitochondria), HSP90 (cytosol), and histone H3 (nucleus). MT, mitochondria; N, nucleus.
(C) Confocal microscopy analysis of SIRT5 localization in control and Sirt5 KD cells along with MitoTracker Red (MT).
