Figure 1. TE inhibits lung colonization and spontaneous metastasis.
The effect of TE on lung colonizing ability of highly metastatic lines 66.1 (A) and line 410.4 (B) was determined. (A) 150µl PBS or TE or (B) 200 µl PBS or TE was injected i.p. daily for 10 days. On day 4, 1 × 105 line 66.1 or 410.4 tumor cells were injected into the lateral tail vein. On day 21 post tumor cell injection, mice were euthanized and surface lung tumor colonies were counted. (C) 5×105 line 410.4 tumor cells were injected subcutaneously proximal to the mammary gland of mice. When tumor became palpable (day +5) 200 µl of either PBS or TE was injected i.p. daily for the next 18 days. Tumor growth was monitored and when tumors measured 18 mm in average diameter or earlier if animals appeared moribund, mice were euthanized and spontaneous lung metastases enumerated. (D) Shows the null effect of TE on tumor growth.