Figure 3. Effect of high and low m.w. TE on cell proliferation and metastasis.

(A) Line 66.1 cells were seeded in 24-well plates and PBS or high molecular weight fraction of TE was added at 6.25, 12.5 and 25 µl doses at time 0. Forty-eight hours later, cell number was determined by MTT assay. (B) 100 µl high m.w. fraction, 200 µl of PBS, stock TE or low m.w. fraction of TE was injected i.p. for 10 days. On day 4 of treatment, 2 × 10⁵ line 66.1 tumor cells were injected i.v. On day 19 post tumor cell injection, mice were euthanized, and surface lung and heart tumor colonies counted. P values by Wilcoxon exact two-sided test at the 0.05 level of significance.