Table 1. PTH-Amino acid yields from IEX fractions.

Automated Edman degradation was performed on an Applied Biosystems 494 HT. Mass spectrometric analysis was performed on a Shimadzu Axima CFR Plus using sinapinic acid as the matrix. Proteins were identified by a similarity search of the nr database using the BLASTP 2.2.24+ algorithm via the NCBI web interface.

Cycle	Called Residues	Yield (pmoles)
1	N L	54.3 66.3
2	G I	53.1 58.7
3	T P	54.9 50.6
4	N F	41.5 49.3