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The main characteristics of IL-1 gene and techniques used for screening.
| IL-1α | IL-1β | |
|---|---|---|
| Polymorphism type | Single base C/T | Single base C/T |
| Polymorphism site | −889 | −511 |
| PCR primers | ||
| Upstream | 5-GGGGGCTTCACTATGTTGCCCA CACTGGACTAA-3 |
5-TGGCATTGATCTGGTTCATC-3 |
| Downstream | 5-GAAGGCATGGATTTTTACATAT GAGCCTTCCATG-3 |
5-GTTTAGGAATCTTCCCACTT-3 |
| PCR condition | ||
| Denaturation | 94°C | 94°C |
| Annealing | 57°C | 55°C |
| Extension | 72°C | 72°C |
| Cycles (n) | 35 | 35 |
| Digestion | NCOI | AvaI |
| Fragment size (pb) | 295 | 304 |
