Figure 7.

Effect of inhaled dead or live A. fumigatus conidia on pulmonary inflammation and airway remodeling in sensitized mice. BALF cells from naïve mice or mice challenged with dead or live A. fumigatus conidia were cytospun and stained with Quick Dip stain for differential analysis. Monocytes/macrophages (a), neutrophils (b), eosinophils (c), and lymphocytes (d) were enumerated for each group at days 3, 7, and 28 after third fungal challenge. The percentages indicated on the bars in (a–d) represent the cells quantified as % of total cells. PAS staining or Gomori's trichome staining was used to measure goblet cell% lining the columnar epithelial cells (e) or subepithelial collagen layer thickness (f). Naïve levels are indicated by the dashed line. Counts from naïve mice are represented by a dashed line (a–f). Bars represent mean ± SEM, n = 4–8 mice/group. * and ^# represent P < 0.05, as compared to naïve mice or mice challenged with dead conidia, respectively.