TABLE 1.
Experimental details of recent MS-based proteomic studies of HDL
| Reference | Patient Population | HDL Type and Separation Technique | MS Approach | MS Database Searched | Gel Separation? |
| (21) | Pooled plasma from healthy adults (n = 4) | HDL2, HDL3 isolated by density gradient UC (KBr) | MALDI-TOF | NCBI and SwissProt | Yes |
| 2D gel | |||||
| (24) | Pool of plasma from >10,000 donors | Total HDL isolated by one-step density gradient UC (KBr) | MALDI-TOF | UniProt | Yes |
| 2D gel | |||||
| (25) | 1 healthy donor | Total HDL isolated by density gradient UC (KBr) | HPLC to separate peptides MALDI- TOF | Details not given | No |
| (22) | Healthy donors (n not reported) | Total HDL isolated by one-step density gradient UC (KBr) and immunoisolation | MALDI-TOF, isotope coded affinity tag plus Western blot analysis | Details not given | Yes |
| 1D and 2D gel | |||||
| (23) | Studies of total HDL: 20 males; studies of HDL3: 6 healthy males and 7 with CAD | Total HDL and HDL3 isolated by density gradient UC (KBr) | LC-ESI-MS/MS | International Protein Index | No |
| (26) | 9 healthy normolipidemic males and 3 samples, each consisting of a pool from 20 healthy normolipidemic males | HDL2b, 2a, 3a, 3b and 3c isolated by density gradient UC (KBr) | LC-ESI-MS/MS | SwissProt | No |
| (33) | 3 healthy normolipidemic males | “HDL” isolated by high resolution size exclusion chromatography followed by lipid removal agent | LC-ESI MS/MS | UniProt and SwissProt | No |
| (27) | 10 healthy adults, 10 with stable coronary disease and 10 with acute coronary syndrome. Age-matched males | Total HDL separated by density gradient UC (KBr) | LC-ESI MS/MS | UniProt | Yes |
| 1D gel | |||||
| (32) | Aged-matched females with rheumatoid arthritis: 4 with anti-inflammatory HDL index, and 4 with proinflammatory HDL index | Total HDL isolated by immunoaffinity capture | LC-ESI MS/MS | UniProt | Yes |
| IEF gel | |||||
| (28) | 19 healthy adults and 27 with end stage renal disease on hemodialysis. Males and females | Total HDL isolated by one-step density gradient UC (KBr) | LC-ESI-MS/MS | NCBI | No |
| (29) | 15 healthy adults and 15 with psoriasis. Males and females | Total HDL isolated by two-step density gradient UC (KBr) | LC-ESI-MS/MS | SwissProt | No |
| (30) | 10 healthy adults and 10 with end stage renal disease. Males and females | Total HDL isolated by density gradient UC (KBr) | LC-ESI-MS/MS | SwissProt | No |
| (31) | 30 healthy adults and 30 with end stage kidney disease on hemodialysis. Males and females | Total HDL isolated by three-step density gradient UC (KBr) | MALDI-TOF and iTRAQ labeling prior to LC-ESI MS/MS | UniProt | Yes |
| IEF gel |
IEF, isoelectric focusing; iTRAQ, multiplexed isobaric-tagged reagents produced by Sciex; UC, ultracentrifugation.