TABLE 2.
Calculation of size and composition of S93 and S80 by volumetric analysis
| In Vitro Particle (Ref.) | Method | A-I | A-I | A-II | A-II | (Ref.) | PL | PL | CE | CE | UC | UC | TG | TG |
| MW | Vol (Å) | MW | Vol (Å) | MW | Vol (Å) | MW | Vol (Å) | MW | Vol (Å) | MW | Vol (Å) | |||
| S93 (Silva, personal communication) | LCAT | 28016 | 33899 | 17414 | 21071 | (27) | 787 | 1307 | 650 | 1179 | 387 | 610 | 850 | 1575 |
| S80 (22) | LCAT+ CETP | 28016 | 33899 | 17414 | 21071 | (27) | 787 | 1307 | 650 | 1179 | 387 | 610 | 850 | 1575 |
| A-I per Particle | A-II per Particle | PL% | PL per Particle | CE% | CE per Particle | UC% | UC per Particle | TG% | TG per Particle |
| 3 | 0 | 40.8 | 96.6 a | 12.9 | 37.0 a | 1.2 | 5.7 a | 0.0 | 0.0 a |
| 2 | 0 | 86 | 12 | 6 | 10 |
| Core Vol (CE+TG) (Å3) b | Core Diameter (Å3) c | Total HDL Vol (Å3) b | HDL Diameter (Å) c | HDL Diameter with Single Hydrated Layer (+5.6 Å) | HDL Diameter With Double Hydrated Layer (+11.2 Å) |
| 43605 | 45 | 275103 | 81 | 86 | 93 |
| 29898 | 39 | 213759 | 74 | 80 | 86 |
Calculation of the number nL of each type of lipid L (PL, CE, UC, TG) per HDL particle either experimentally determined or by examining different combinations of the number nA of each type of apolipoprotein A (A-I, A-II…) per HDL particle is as follows: nL = (MWP/ MWL) × (%L/ %P), where MWP = total molecular weight of protein = (A-I MW × nA-I) + (A-II MW × nA-II) + …), where MWL = molecular weight of single lipid L; %L = weight percentage of L; %P = weight percentage of total apolipoprotein = (1 − ∑%L).
Calculation of core volume (VC) = (nCE × VCE) + (nTG × VTG), where VL is volume of each L. Calculation of the total HDL volume (VHDL) = ∑ (nL × VL) + VP, where VP is total volume of apolipoprotein.
Calculation of core and HDL diameters = 2r; r = (3V/(4π))1/3 assuming a sphere.