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. 2013 Oct;54(10):2874–2883. doi: 10.1194/jlr.M042499

Fig. 1.

Fig. 1.

Enzymatic activity of recombinant 11β-HSD1 from various species was determined in lysates of transfected HEK-293 cells as described in Materials and Methods. (A) Stereoselective oxidoreduction of 7-oxoLCA (1 µM) to CDCA (black bars) and UDCA (white bars) by 11β-HSD1 from various species after incubation for 10 min at 37°C. (B) Guinea-pig liver microsomes were incubated for 40 min with 7-oxoLCA (1 μM) and glucose-6-phosphate (1 mM). Bile acids were extracted and quantitated by LC-MS/MS. Unconverted 7-oxoLCA is represented by black bars. Data (n = 3) represent mean ± SD. (C) Oxidoreduction of cortisone by guinea-pig liver microsomes. (D) Western blotting against 11β-HSD1 in guinea-pig (GPLm) and mouse liver microsomes (MLm).