. 2013 Oct;54(10):2898–2908. doi: 10.1194/jlr.P035808

TABLE 2.

Conditions for tandem mass spectrometry analysis of lipid species identified in human plasma

Lipid Class or Subclass	No. of Species	Internal Standard	Pmol^a	Parent Ion	Experiment	Voltage Settings (V)
Lipid Class or Subclass	No. of Species	Internal Standard	Pmol^a	Parent Ion	Experiment	DP	EP	CollE	CXP
dhCer	6	dhCer 8:0	100	[M+H]⁺	PIS, m/z 284.3	90	30	28	10
Cer	6	Cer 17:0	100	[M+H]⁺	PIS, m/z 264.3	50	10	35	12
MHC	6	MHC 16:0 d₃	50	[M+H]⁺	PIS, m/z 264.3	77	10	50	12
DHC	6	DHC 16:0 d₃	50	[M+H]⁺	PIS, m/z 264.3	100	10	65	12
THC	6	THC 17:0	50	[M+H]⁺	PIS, m/z 264.3	130	10	73	12
GM	6	THC 17:0	50	[M+H]⁺	PIS, m/z 264.3	155	10	105	16
SM	19	SM 12:0	200	[M+H]⁺	PIS, m/z 184.1	65	10	35	12
PC	41	PC 13:0/13:0	100	[M+H]⁺	PIS, m/z 184.1	100	10	45	11
PC(O)	18	PC 13:0/13:0	100	[M+H]⁺	PIS, m/z 184.1	100	10	45	11
PC(P)	8	PC 13:0/13:0	100	[M+H]⁺	PIS, m/z 184.1	100	10	45	11
LPC	21	LPC 13:0	100	[M+H]⁺	PIS, m/z 184.1	90	10	38	12
LPC(O)	6	LPC 13:0	100	[M+H]⁺	PIS, m/z 285.2	90	10	42	5
PE	18	PE 17:0/17:0	100	[M+H]⁺	NL, 141 Da	80	10	31	7
PE(O)	12	PE 17:0/17:0	100	[M+H]⁺	NL, 141 Da	80	10	31	7
PE(P)	9	PE 17:0/17:0	100	[M+H]⁺	NL, 141 Da	80	10	31	7
LPE	6	PE 14:0/0:0	100	[M+H]⁺	NL, 141 Da	80	10	31	7
PI	17	PE 17:0/17:0	100	[M+NH₄]⁺	PIS, m/z 184.1	51	10	43	14
PS	7	PS 17:0/17:0	100	[M+H]⁺	NL, 185 Da	86	10	29	16
PG	4	PG 17:0/17:0	100	[M+NH₄]⁺	NL, 189 Da	60	10	25	12
CE	26	CE 18:0 d₆	1,000	[M+NH₄]⁺	PIS, m/z 369.3	30	10	20	12
COH	1	COH d₇	1,000	[M+NH₄]⁺	PIS, m/z 369.3	55	10	17	12
DG	21	DG 15:0/15:0	200	[M+NH₄]⁺	NL, fatty acid	55	10	30	22
TG	43	TG 17:0/17:0/17:0	100	[M+NH₄]⁺	NL, fatty acid	95	10	30	12

PIS, precursor ion scan; NL, neutral loss scan; DP, declustering potential; EP, entrance potential; CollE, collision energy; CXP, collision cell exit potential.

Amount of internal standard per sample.