TABLE 3.

Assay performance of lipid classes and subclasses from plasma QC samples

Lipid Class or Subclassa	Linear Range (μM)b	R2	Recovery, %c	Intra-run Variation, %d	Assay Variation, %e
dhCer	0.005–100	0.990	89.6	9.9	13.1
Cer	0.005–100	0.996	91.8	7.2	6.8
Monohexosylceramidef	—	—	88.4	8.5	13.6
DHC	0.01–100	0.999	91.3	9.2	9.6
THC	0.01–100	0.999	89.6	8.8	9.6
GMf	—	—	—	8.3	9.1
SM	0.005–500	0.988	90.4	5.4	6.4
PC	0.005–500	0.988	89.6	7.7	8.3
PC(O)f	—	—	—	6.9	8.9
PC(P)f	—	—	—	5.7	9.4
LPC	0.01–100	0.999	90.6	7.9	14.3
LPC(O)f	—	—	—	6.0	6.4
PE	0.005–100	0.994	88.2	5.5	6.3
PE(O)f	—	—	—	6.2	7.4
PE(P)	0.005–100	0.994	—	7.4	7.8
LPE	0.005–100	0.994	87.7	7.4	9.8
PIf	—	—	—	6.9	8.3
PSf	—	—	89.1	22.4	22.6
PG	0.01–100	0.998	89.1	13.2	13.8
CE	0.005–1000	0.998	86.3	12.4	13.2
COH	5–100	0.999	83.7	17.9	17.5
DG	0.05–100	0.996	92.3	20.1	17.5
TG	0.05–1000	0.997	93.3	23.7	20.4

^aSum of the individual species within that class or subclass.

^bCalculated from one lipid species (spiked into QC plasma) and compared with the internal standard for that class or subclass.

^cPercent recovery compared with matrix spike.

^dAverage intra-run CV from four separate analyses.

^eCV from 63 QC plasma samples across 1,076 samples in four analytical runs.

^fNo suitable standards were available.