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. Author manuscript; available in PMC: 2014 Jul 18.
Published in final edited form as: Cell. 2013 Jul 11;154(2):442–451. doi: 10.1016/j.cell.2013.06.044

Figure 2. CRISPRi is highly specific in human cells.

Figure 2

(A) RNA sequencing RPKM (reads per kilo base per million) are plotted for GFP+ HEK293 cells stably expressing dCas9-KRAB and either a negative control sgRNA targeting Gal4 or an sgRNA targeting GFP. Total RNA was collected 15 days following lentiviral transduction. The data are representative of 2 independent biological replicates. See also Figure S3. (B) A histogram showing the fold changes in gene expression from (A) plotting sgGFP-NT1 over the negative control. The GFP is indicated with an arrow. The data are representative of 2 independent biological replicates. See also Figure S3.