Skip to main content
. Author manuscript; available in PMC: 2014 Jul 18.
Published in final edited form as: Cell. 2013 Jul 11;154(2):442–451. doi: 10.1016/j.cell.2013.06.044

Figure 4. CRISPRi can suppress gene expression from the promoter of a gene.

Figure 4

(A) A dCas9-KRAB fusion protein efficiently silences GFP expression when targeted to the SV40 promoter of an SV40-GFP reporter. 6 sgRNAs targeting different regions of the SV40 promoter as indicated are transfected into GFP+HEK293 cells stably expressing either dCas9 (upper panel) or dCas9-KRAB (lower panel). GFP fluorescence is quantified by flow cytometry 6 days following transfection and displayed as signal normalized to a vector control. The data are displayed as mean ± standard deviation for 3 independent experiments. (B) The dCas9 construct and an sgRNA (sgTET) construct were co-transformed into a yeast strain expressing a TetON-Venus reporter and the rtTA protein. Doxycycline was added to cells expressing rtTA alone or rtTA, dCas9 and sgTET. The data are displayed as mean ± standard deviation for 3 independent experiments.