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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: J Pept Sci. 2013 Feb 25;19(4):10.1002/psc.2499. doi: 10.1002/psc.2499

Figure 3.

Figure 3

The relative cellular presence of pERK after stimulation with endothelin-1 of stably transfected CHO cells expressing the (A) endothelin A (CHO-ETA); or (B) endothelin B (CHO-ETB) receptors and either E3A or E3B C-terminal peptides. The level of pERK is shown relative to the control CHO-ETA or CHO-ETB cells, transfected with an empty vector (Emp Vec) not expressing the peptides. All tested cell cultures were treated with endothelin-1 at 10 nM (a maximal stimulatory dose) for 5 min (optimum incubation time). Blots are representative of three independent experiments. Bar graphs represent the mean value of relative ERK activation compared with the control (empty vector) cells. Error bars represent the standard deviation. Data were evaluated for statistical significance by use of a student's t-test. * = P < 0.05 versus Emp Vec.