Table 2. Summary of top-scoring categories in DAVID pathway analysis.

Pathway analysis was performed using the DAVID (http://david.abcc.ncifcrf.gov/) [25] suite of tools; adjusted p-values (p_adj) refer to p-values obtained after Benjamini-Hochberg correction. All analyses were performed with the default settings and classification stringency at “medium”.

A: Folinic acid-supplemented wildtype vs. wildtype
increase			decrease
GO term	padj	genes	GO term	padj	genes
lipid metabolic process	0.011	29	ribonucleoprotein complex	4.3×10-06	30
			structural constituent of ribosome	2.5×10-05	11
			mRNA metabolic process	6.4×10-04	20
			vasculature development	0.016	15

B: Folinic acid-supplemented Folr4 mutant vs. Folr4 mutant
increase			decrease
GO term	padj	genes	GO term	padj	genes
immune system process	1.9×10-26	115	structural constituent of ribosome	2.5×10-59	83
leukocyte activation	1.5×10-09	38	cell cycle	1.5×10-10	83
T cell activation	1.3×10-06	23	m-phase	2.3×10-10	51
regulation of immune response	2.2×10-09	35	mitochondrion	2.6×10-10	141
cytokine binding	4.8×10-06	20	oxidative phosphorylation	0.064; 5.8×10-14	20
			mitochondrial lumen	3.5×10-03	22

C: Folr4 mutant vs. wildtype
increase			decrease
GO term	padj	genes	GO term	padj	genes
structural constituent of ribosome	1.7×10-21	36	defense response	8.7×10-05	25
mitochondrion	0.1	33	immune response	1.8×10-07	31

D: Folinic acid-supplemented Folr4 mutant vs. Folr4 mutant compared to Folr4 mutant vs. wildtype
increase			decrease
GO term	padj	genes	GO term	padj	genes
immune system process	6.6×10-06	24	structural constituent of ribosome	2.4×10-29	36
			mitochondrion	0.036	33
			glutathione transferase activity	0.0054	6