Table 3. Folinic acid-mediated reversal of gene expression changes in Folr4 mutant mice.

We observed 562 gene probes that appear to reverse the expression change elicited by the Folr4 mutation when supplemented with folinic acid. Eight genes were chosen for validation by qPCR: four genes that were initially up-regulated in the mutant vs. the wildtype, yet showed a decrease in expression in folinic acid-supplemented mutants, and four genes with the opposite pattern of gene expression changes: reduced expression in mutant compared to wildtype, and increased expression in folinic acid supplemented mutant compared to unsupplemented mutant. In all cases, the direction of the expression change could be confirmed by qPCR, although not all qPCR assays reached statistical significance in a t-test.

	Microarray				qRT_PCR
	KO vs WT		FA_KO vs KO		KO vs WT		FA_KO vs KO
Gene Symbol	Fold change	p-value	Fold change	p-value	Fold change	p-value	Fold change	p-value
Fst	2.92	0.042	-2.98	0.048	3.31	0.018	-3.28	0.130
Pcx	1.85	0.009	-2.56	0.035	2.13	0.042	-5.03	0.026
Tpd52l1	1.67	0.008	-2.07	0.003	1.62	0.023	-1.49	0.296
Vit	1.82	0.041	-1.96	0.037	1.68	0.032	-2.04	0.003
Ccr2	-2.19	0.017	2.55	0.012	-1.98	0.002	2.27	0.028
Fnip1	-1.47	0.046	1.91	0.024	-1.40	0.118	1.34	0.036
Slfn5	-1.84	0.042	1.81	0.047	-1.53	0.021	1.24	0.361
Tmem173	-1.65	0.009	2.49	0.025	-1.30	0.156	1.87	0.042