Abstract
The use of a Spinco L-4 zonal centrifuge with the B-XVI continuous-flow rotor for the purification of canine distemper and infectious canine hepatitis viruses is described. Up to 68 liters of virus was processed at one time. Infectious canine hepatitis virus was found to band at 39% sucrose and canine distemper virus banded between 32 and 48% sucrose. The virus was concentrated 10-fold, and the purity of the virus, as measured by protein concentration, was increased by 99%.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anderson N. G., Barringer H. P., Amburgey J. W., Jr, Cline G. B., Nunley C. E., Berman A. S. Continuous-flow centrifugation combined with isopycnic banding: rotors B-8 and B-IX. Natl Cancer Inst Monogr. 1966 Jun;21:199–216. [PubMed] [Google Scholar]
- Cline G. B., Nunley C. E., Anderson N. G. Improved continuous flow centrifugation with banding. Nature. 1966 Oct 29;212(5061):487–489. doi: 10.1038/212487a0. [DOI] [PubMed] [Google Scholar]
- Fox S. M., Birnie G. D., Martin E. M., Sonnabend J. A. Isolation of polyoma virus and Semliki Forest virus from tissue culture fluid by continuous flow zonal ultracentrifugation. J Gen Virol. 1967 Oct;1(4):577–580. doi: 10.1099/0022-1317-1-4-577. [DOI] [PubMed] [Google Scholar]
- Gerin J. L., Richter W. R., Fenters J. D., Holper J. C. Use of zonal ultracentrifuge systems for biophysical studies of rhinoviruses. J Virol. 1968 Sep;2(9):937–943. doi: 10.1128/jvi.2.9.937-943.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Reimer C. B., Baker R. S., Van Frank R. M., Newlin T. E., Cline G. B., Anderson N. G. Purification of large quantities of influenza virus by density gradient centrifugation. J Virol. 1967 Dec;1(6):1207–1216. doi: 10.1128/jvi.1.6.1207-1216.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]