FIG. 4.

Immunofluorescence staining of β-secretase (BACE) protein in three groups. (A) Samples were fixed, stained with an antibody against BACE followed by a rhodamine-conjugated secondary antibody (red) and analyzed by fluorescence microscopy. Nuclei were visualized by 4′,6-diamidino-2-phenylindole (DAPI) staining (blue) and are presented in merged images (magnification, ×200). (B) The percentage of BACE-positive cells in the sham, normothermia, and hypothermia groups were calculated. All data are expressed as mean±SD (n=10). *p<0.05, **p<0.01 versus the sham group; ^#p<0.05 versus the normothermia group. Color images available online at www.liebertpub.com/ther