(A) The proliferative response of human CD4+ T cells (n = 3) to WT and GTKO porcine aortic endothelial cells (pAECs) before and after activation by pIFN-γ (left), where the response was significantly less to GTKO pAECs before (P < 0.001) and after (P < 0.01) activation. Additionally, MLRs were harvested on three consecutive days (4, 5, and 6) where CD4+ T-cell proliferation was consistently lower in response to GTKO pAECs. 3H incorporation values are presented as counts per minute (CPM). Data represent the mean (±SEM) and are representative of three different experiments. (B) The proliferative response of human CD8+ T cells (n = 3) to WT and GTKO pAECs before and after activation by pIFN-γ (left), where the response was significantly less to GTKO pAECs before (P < 0.001) and after (P < 0.05) activation. Additionally, MLRs were harvested on three consecutive days (4, 5, and 6) where CD8+ T-cell proliferation was consistently lower in response to GTKO pAECs. 3H incorporation values are presented as CPM. Data represent the mean (±SEM) and are representative of three different experiments.