Table 3. Total NO× (nitrate/nitrite) concentration in locus coeruleus tissue homogenates from animals treated with clonidine (60 μg/kg, i.v.), ethanol (1 g/kg, i.v.), NOS inhibitor, or vehicle.

Values are expressed as μmol/g protein (mean ± S.E.M.).

Treatment	Total NO× Concentration in LC Homogenates
Saline (i.v.)	1.96 ± 0.08 (6)
Clonidine	2.28 ± 0.26 (6)
Ethanol	2.42 ± 0.57 (6)
Clonidine + Ethanol	2.67 ± 0.33 (6)
aCSF (i.c.) + Saline (i.v.)	2.57 ± 0.32 (5)
L-NAME (0.5 mg, i.c.) + Saline (i.v.)	3.24 ± 0.50 (5)
NPLA (1 μg, i.c.) + Saline (i.v.)	2.48 ± 0.44 (5)
L-NIO (10 μg, i.c.) + Saline (i.v.)	2.89 ± 0.31 (5)

N values are indicated in parentheses. There were no significant treatment differences in NO× level of LC homogenates (one-way ANOVA).