Figure 2.

Nuclear Yes-associated protein (Yap) does not rescue apoptosis at high cell density. (a) MCF10A cells expressing Flag-Yap1, Flag-Yap1 S127A or vector control were plated sparse and dense, and irradiated at 20 Gy. After 24 h, cells were fixed for immunofluorescence analysis with the anti-Flag antibody. Nuclear staining is denoted by Hoechst 33342. Panels show representative fields of three independent experiments. Arrows indicate some of the cells with nuclear-localized Yap1. (b) The cells used in (a) were plated sparse and dense, irradiated at 20 Gy and harvested for FACS analysis 120 h post-IR. Percentages of cells in the different fractions (sub-G1, G1, S and G2-M) are indicated