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The recruitment of ubiquitin and LC3 to GFP-Gal3-positive damaged lysosomes. (A–C) Atg7+/+ and Atg7–/– MEFs stably expressing GFP-Gal3 (A, C) or GFP-Ub (B) were treated with 1000 μM LLOMe or 250 μg/ml silica for 3 h. Cells were subjected to immunocytochemistry using the following antibodies: anti-LC3 and anti-Lamp1 (A), anti-p62 and anti-Lamp1 (B), or anti-FK2 and anti-p62 (C). Bars: 10 μm.
