Figure 5.

Autophagy-deficient renal tubules exhibit severe injury and dysfunction under hyperuricaemia. (A) Plasma creatinine and urea nitrogen in Atg5^F/F and Atg5^F/F;KAP mice treated with vehicle or UA+OA. The values displayed represent means±s.e. Statistically significant differences (*P<0.05) are indicated. F/F: Atg5^F/F mice; F/F;KAP: Atg5^F/F;KAP mice. (B, C) PAS-stained renal cortical region of Atg5^F/F and Atg5^F/F;KAP mice treated with vehicle or UA+OA (n=4–7). Bars: 40 μm (B). Kidney injury score of Atg5^F/F and Atg5^F/F;KAP mice treated with vehicle or UA+OA (C). (D) Kidney cortexes from Atg5^F/F and Atg5^F/F;KAP mice treated with vehicle or UA+OA were subjected to immunohistocheminal analysis of Lamp1 and LC3. Green: LC3; magenta: Lamp1; blue: DAPI. F/F: Atg5^F/F mice; F/F;KAP: Atg5^F/F;KAP mice. Bar: 5 μm. (E) Electron micrographs of kidney proximal tubules in Atg5 ^F/F and Atg5^F/F;KAP mice treated with UA+OA. Yellow arrowhead: autophagosome; asterisk: lysosome; Mt: mitochondrion. Bar: 1 μm. (F) Model of autophagic clearance of damaged lysosomes.

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