Figure 7.

Mfn2 regulates PERK activity. (A) Immunodetection of p-PERK and p-eIF2α in Mfn2 KO MEFs, Mfn2 knockdown 3T3-L1 fibroblasts, and Mfn2 knockdown C2C12 myoblasts. (B) Densitometric quantification of p-PERK and p-eIF2α in MEFs. Data are mean±s.e.m. (n=3). *P<0.05 versus WT cells. (C, D) Immunodetection of p-eIF2α, eIF2α, CHOP, XBP-1s, LC3b, and p62 in Mfn2-deficient liver (C) or skeletal muscle (D) from tissue-specific KO mice. (E) Co-immunoprecipitation of Mfn2 and PERK in WT or Mfn2 KO cells stably expressing PERK-myc or an empty vector (pBABE-myc; negative control). (F) Co-immunoprecipitation of endogenous Mfn2 and PERK in WT MEF lysates. PERK was immunoprecipitated with a C-terminal antibody and co-immunoprecipitation of Mfn2 was detected by western blot.

Source data for this figure is available on the online supplementary information page.

Source Data for Figure 7 ^{(830.8KB, pdf)}