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. Author manuscript; available in PMC: 2013 Sep 11.
Published in final edited form as: J Mol Biol. 2011 Nov 12;415(2):248–262. doi: 10.1016/j.jmb.2011.11.014

FIG. 5. Donor template degradation of the pre-drug RT, post-drug RT, and post-drug ΔSGRT.

FIG. 5

(A) 32P labeled donor RNA 80 nt template was annealed to cold 20 nt DNA primers at a 1:2 ratio respectively. Pre-drug RT, post-drug RT, and post-drug ΔSG RT was pre-incubated with the T/P complex for 3 min before the addition of 6 mM/dNTP 5 μM for initiating the reaction. Reactions were quenched with EDTA in the following time course assay at (0.5, 1, 3, 15, 30 minutes). Donor T: 32P labeled donor RNA 80 nt template. The RT activities were similar to Fig. 3.