(A) 32P labeled donor RNA 80 nt template was annealed to cold 20 nt DNA primers at a 1:2 ratio respectively. Pre-drug RT, post-drug RT, and post-drug ΔSG RT was pre-incubated with the T/P complex for 3 min before the addition of 6 mM/dNTP 5 μM for initiating the reaction. Reactions were quenched with EDTA in the following time course assay at (0.5, 1, 3, 15, 30 minutes). Donor T: 32P labeled donor RNA 80 nt template. The RT activities were similar to Fig. 3.