Table 1.

Determining the K_DK_offK_on of pre-drug RT and post-drug RT

	KDa	Koffb	Konc
Pre-drug RT	5.9 × 10−7 M ± 0.0616	6.1 × 10−4s−1 ± 0.0105	1.9 × 104 M−1s−1
Post-drug RT	7.8 × 10−8 M ± 0.2761	3.1 × 10−4 ± 0.0167	3.5 × 105 M−1s−1
Post-drug ASG RT	1.7 × 10−7 M ± 0.2560	2.6 × 10−4 s−1 ± 0.0217	1.5 × 105 M−1s−1

^aDNA binding study summarized. The K_D of each RT isolate was extrapolated from a sigmoid binding curve.

^bK_off was calculated from the exponential decay equation (Y=e^−Koff(t)) where Y=relative rate of incorporation and t=time. Each data point represents value from three independent experiments with standard error.

^cQuantification of the percent bound DNA of all three RT protein isolates. K_on rate of the three RTs were calculated using the equation K_D = K_off/K_on.