Fig. 1.

A diagram of the polymorphic PCR band pattern of 20 wild Oryza species obtained using 22 INDEL markers. The color pattern indicates how many polymorphic bands were detected in each of 22 INDEL markers for 20 wild Oryza species. The species indicated with a same color exhibited an identical band size for each marker on the gel electrophoresis. The number in each color indicates the estimated band size (bp). For example, the Ch7-233W marker gave rise to 5 different bands: 281 bp (magenta), 440 bp (yellow), 200 bp (green), 300 bp (light blue) and 181/350 bp (dark blue). In BBCC and CCDD tetraploid species, this marker gave two bands from both BB and CC types, colored green and light blue. Two numbers with a slash (ex., 181/350) indicate that in tetraploid species (for example, HHJJ), the genome types from which both bands were raised are uncertain. Two numbers with a comma (ex., 210, 370) indicate that the marker amplifies two PCR bands in a diploid species. The numbers with asterisks indicate that those bands are polymorphic in several accessions within the same species. ruf, rufipogon; bar, barthii; glu, glumaepatula; mer, meridionalis; lon, longistaminata; pun, punctata; min, minuta; off, officinalis; eic, eichingeri; rhi, rhizomatis; lat, latifolia; alt, alta; gra, grandiglumis; aus, australiensis; bra, brachyantha; gta, granulata; mey, meyeriana; rid, ridleyi; log, longiglumis. The seven marker IDs colored red are a minimum marker set useful for classifying species and genome species in the genus Oryza.