Table 2.

PCR primer sequences used for the amplification of wheat AP2 homoeologs

Target	Primer name	Sequence of primer (5′-3′)	Primer name	Sequence of primer (5′-3′)	PCR mixture			Tm (°C)	size (kb)
					Buffer	MgCl2 (mM)	DMSO (%)
AP2-A_fragment 1	U1005A23	GCAGACCAGAGAGAGGCTAGAGG	2223L20	CTGCAAGGCCAATTACAGGT	0.2 × GC Buffer II	2.5	8	57	1.2
AP2-A_fragment 2	F695	TGCGGCAAGCAGGTCTATCTG	A3794L19	CCCATGCTCCTCCGTGATC	1 × Ex Taq polymerase buffer	2.0	8	65	2.0
AP2-B	F-est2	AGAGCAGGGCAGAGGGAGGCGTAGGG	R-est1543	GCTGGCTGCTCTCGACGGATGGT	1 × Ex Taq polymerase buffer	2.0	8	65	2.8
AP2-D_fragment 1	55U24	GCAAGCAGGGAGGGGAGCTAGCCA	R1690	GGCTCGAACTCCTCGGCG	1 × Ex Taq polymerase buffer	2.5	12	65	1.8
AP2-D_fragment 2	F695	TGCGGCAAGCAGGTCTATCTG	3897L20	TGGAGCTGGTCTTGATGGTC	1 × Ex Taq polymerase buffer	2.5	8	65	2.0