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. 2013 Sep 11;8(9):e74662. doi: 10.1371/journal.pone.0074662

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© 2013 Verma et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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H1 variants were cloned into a Flag epitope vector and transfected with a vector containing LANA-myc. Histone H2B-Flag was also transfected for comparison. Anti-myc IP to precipitate LANA co-precipitated H1.c as well as H1.x, the binding affinity of H1.c relatively stronger then H1.x. LANA in the input and IP lanes were detected in anti-myc blot.