Figure 7. Adiponectin does not localize with Lamp1 containing membranes in adipocytes.

Control (uninfected) 3T3L1 cells or cells expressing shRNAs for FIP1 were cultured and differentiated to adipocytes and used on day 10 after differentiation. Cells were fixed, permeabilized and stained with specific antibodies to detect endogenous adiponectin or lamp1. Secondary antibodies conjugated to Alexa488 and Alexa594 were used to visualize the proteins. Representative cells are shown from 3 independent experiments. Panels c, and f show the merged images.