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. 2013 Sep 11;8(9):e71832. doi: 10.1371/journal.pone.0071832

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© 2013 Rudolf et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Detailed species composition at the 4 sampling sites of the Rhine-Main metropolitan area (right graph) and 5 sampling sites at Hamburg metropolitan area (left graph). DNA samples from single individuals collected at these sampling sites were subjected to the multiplex real-time PCR and analysed for the presence of biotype hybrids. Bars represent species distribution in percent at each site. White indicates Cpp. biotype pipiens; red Cpp. biotype molestus; yellow hybrids of biotypes pipiens and molestus. Biotype hybrids were found at Dirmstein and Bobenheim-Roxheim trapping sites in Southwest Germany and at the Wulksfelde trapping site in northern Germany. Additionally, the presence of Cx. torrentium was assed and is indicated by the grey bars in both graphs.