Figure 2. Lack of rearward surface flow in crawling cells.

Two cAR1-paGFP expressing cells in which the front (A) or lateral side near the front (B) has been photoactivated crawling toward a cAMP source. Fluorescence and DIC images taken as in Figure 1. From left to right: before photoactivation, immediately after activation and 1, 2 or 3 minutes later.