Figure 4. Interaction of Mypt1 and PP1β isoforms.

(A) A schematic of Mypt1 constructs used in immunoprecipitation experiments. The sequence of the PP1 binding domain is indicated as the WT KVKF, the partial-loss-of-function KMKF and the null KAKA. (B) Myc-tagged Mypt1 and GFP-tagged PP1β proteins were expressed in HEK293T cells and immunoprecipitated with anti-myc antibodies. The IPs were immunoblotted with anti-GFP antibodies and anti-myc antibodies.