Figure 4. Effect of ECM collagen on cell cycle progression in LSMCs.

Leiomyoma cells were treated with serum free (SF) medium or PDGF (10ng/ml) for 24 hours on either plastic or monomeric collagen matrices and then assayed by FACs analysis for cell cycle phase. 10,000 events were counted per cell line and the percent of cells in each phase is expressed as total number of diploid cells. Statistical analysis by orthogonal contrast statements was performed between treatments in the S phase of the cellular cycle to address changes in cellular proliferation. Asterisks denote statistical significance of the indicated comparisons (n=6; p<0.05).