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. 2013 Sep 11;8(9):e75844. doi: 10.1371/journal.pone.0075844

Figure 8. Activation of MAPK signaling pathway is influenced by the interaction of LSMCs with ECM.

Figure 8

(A & B) LSMCs were cultured on plastic, monomeric or fibrillar collagen-coated dishes and then treated with 10 ng/ml PDGF for various time points. Lysates were blotted for (A) pERK1/2 and ERK1/2, and (B) pPDGFR and PDGFR. Densitometry values were normalized to total protein expression for each phosphorylated protein. (C) LSMCs cultured on plastic dishes were pre-treated with 10 µM of AG1296 or 25 µM of PD98059 in serum-free medium for two hours followed by treatment with 10 ng/ml PDGF for 10 minutes. Lysates were blotted for pERK1/2 and ERK1/2 Statistical significance within each matrix and compared to non-treated sample is indicated by asterisks. Letters indicate statistical differences across all samples on different matrices (n =3, p<0.05).