Table 2. Sequence reads from specimen 1 giving BLAST hits with an E value ≤10^–6.

Library/database	Read length (nucleotides)	Read ID	E value	Matches	Database entry
Non-destructive extraction, complete database	76	HRA5KJN01BI264	8×10–19	51/51 and 31/31a	Bombus hypocrita mitochondrial cytochrome oxidase subunit I gene
	43	HRA5KJN01ANAS1	2×10–6	33/34	Burkholderia sp. genome
Non-destructive extraction, Apidae database	76	HRA5KJN01BI264	8×10–19	51/51 and 31/31a	Bombus hypocrita mitochondrial cytochrome oxidase subunit I gene
Destructive extraction, complete database	459	HRA5KJN01BDMRJ	5×10–98	385/452	Bradyrhizobium japonicum genome
	98	HRA5KJN01BP4B0	3×10–16	59/63	Xanthomonas oryzae, X. campestris genomes
	53	HRA5KJN01A8GD4	2×10–15	45/45	Gordonia polyisoprenivorans genome
	178	HRA5KJN01AXXA2	10–13	49/51b	Mesorhizobium loti genome
	178	HRA5KJN01AXXA2	10–13	55/59b	Thiobacillus denitrificans genome
	157	HRA5KJN01B26IX	6×10–12	55/60 or 58/64c	Ralstonia solanacearum genome
	53	HRA5KJN01A73N7	2×10–9	35/35	Burkholderia sp. genome
	38	HRA5KJN01BZ4HK	9×10–8	32/32	Stenotrophomonas maltophilia genome
Destructive extraction, Apidae database	45	HRA5KJN01A1IJM	2×10–07	18/18	Bombus terrestris mRNA sequence
	516	HRA5KJN01BVPD4	9×10–06	17/17	Ceratina propinqua elongation factor 1 alpha gene
	40	HRA5KJN01BPOO5	8×10–06	16/16	Apis mellifera proteasome activator complex subunit gene
	46	HRA5KJN01ASAM2	1×10–06	28/29	Apis mellifera genome

See Table S1 for sequence reads.

^aTwo overlapping segments of this read gave hits to non-contiguous regions of the Bombus hypocrita gene.

^bThe same sequence gave hits with identical E values to both Mesorhizobium loti and Thiobacillus denitrificans.

^cThe alignment is slightly different for different strains of Ralstonia solanacearum.