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. 2013 Sep 11;8(9):e73882. doi: 10.1371/journal.pone.0073882

Figure 2. Cellular/extracellular level of SUN proteins during growth of liquid cell cultures in glucose YED medium.

Figure 2

Protein amounts in cell lysates (CELL) and in extracellular extracts (EXTRA), respectively, prepared from liquid cell cultures of BY-Uth1p-HA, BY-Sun4p-HA and BY-Sim1p-HA strains. The standardized amounts of lysate-proteins or the corresponding amounts of extracellular extracts were loaded onto the gel (loading controls see Figure S3 B). A representative Western blot of the 2 independent biological replicates is shown. The highest amount of the particular protein in each immunoblot quantified by densitometry was set as 100%. Growth curves of individual strains are shown (right axis). The value of Mw at the right side of immunoblots represents Mw of particular SUN protein linked to the HA tag.